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Do cryopreserved embryos in liquid nitrogen tanks require regular replacement of liquid nitrogen?

2023-12-18 10:55:18

Samples such as embryos are often stored for a long time, whether in laboratories, hospitals, sample banks, etc.

Liquid nitrogen tanks are commonly used for long-term storage. The treated embryos are soaked in -196°C liquid nitrogen. This low temperature is used to temporarily determine life activities.

Since embryos are precious, no accidents are allowed no matter how they are used. Some novices worry that the liquid nitrogen in which they are soaked will contain impurities after a period of use. They consider replacing the liquid nitrogen regularly to ensure the purity of the storage environment. Then there is this Is it necessary?

Do cryopreserved embryos in liquid nitrogen tanks require regular replacement of liquid nitrogen?

In fact, there is no need!

From the characteristics of liquid nitrogen, it is volatile. It also has a small daily loss when placed in a vacuum insulated liquid nitrogen container. It usually evaporates completely in a few months. If you happen to use a small-capacity large-diameter liquid nitrogen tank to store embryos, it may evaporate in a few weeks. There is no chance that impurities will precipitate if the liquid is stored for too long, threatening the safety of the embryos.

Because it will not be stored in a liquid state for a long time at all, it will only become less and less as time goes by, vaporize and then dissipate into the air, even if a vacuum liquid nitrogen tank is used as a container. Therefore, it is not necessary to replace the liquid nitrogen regularly. What is needed is for the staff to replenish the liquid nitrogen regularly.

In addition, even from the perspective of embryo managers, if you want to provide a purer environment for the samples, you do not need to always look at the naturally evaporating liquid nitrogen, because its low temperature can kill some bacteria. It is better to consider regular canning. Clean the inside of the liquid nitrogen tank before placing the embryos to ensure that the injected liquid nitrogen does not contain impurities.

For subsequent replenishment, consider a self-pressurized liquid nitrogen supply tank. The liquid nitrogen is stored in the tank and transported through pipelines, and does not come into contact with external moisture or impurities.

To store embryo tanks that will be used for a long time, temporarily transfer the embryos to other liquid nitrogen containers every few months, and clean the original tanks before putting them in.

In summary, cryopreserved embryos in liquid nitrogen tanks do not require regular replacement of liquid nitrogen, but the liquid nitrogen tank can be cleaned regularly!

Do cryopreserved embryos in liquid nitrogen tanks require regular replacement of liquid nitrogen