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Do liquid nitrogen tanks used for cell storage need to be filled with liquid nitrogen in advance?

2024-01-17 09:55:00

In major scientific research institutes and university laboratories, liquid nitrogen tanks used for cell storage have become commonplace;

Its vacuum structure uses -196°C liquid nitrogen to cool down, which is exactly what is needed for long-term cell storage during the experiment!

Before use, laboratory personnel need to determine the capacity and diameter of the liquid nitrogen container, how to store it, what accessories are needed, whether it is a freezing rack or a round bucket, and whether to install an intelligent liquid level monitor or a mobile small Carts and more. We also need to consider the issue of fluid replenishment. Should we prepare replenishment tools? Or should we make an agreement with the gas company?

In addition, there is another question that newbies have: Do liquid nitrogen tanks used for cell storage need to be filled with liquid nitrogen in advance?

do you need?

needs!

Although the experimental cells have been processed, some of them were even transferred out after being pre-cooled in a -80°C low-temperature refrigerator. They are already “dormant” or their life activities have slowed down. But after all, it is a biological sample with high vitality requirements. When placing it in a liquid nitrogen tank, you must pay attention to the liquid nitrogen level. It must cover the cells, not to mention that it will be exposed to some air heat transfer.

If there is no liquid nitrogen inside the liquid nitrogen tank used for cell storage at this time, it means that the temperature of the container must not have dropped below -130℃-150℃, or even normal temperature. Put the cells that have been exposed to a little heat into such a temperature. , it is like putting ice cubes into hot water, and the activity of cells cannot be guaranteed at all. Even if liquid nitrogen is injected quickly, it will take a certain amount of time for the normal temperature cell storage tank to drop to close to -196°C, and the cells cannot afford to wait for this cooling process, and they cannot wait!

Therefore, since cells are used for storage, the purpose is to store them statically for a long time to ensure their vitality, and then use them for scientific research experiments. It is necessary to create a comfortable environment in advance. Liquid nitrogen must be put into the liquid nitrogen tank used for cell storage in advance. of!

Do liquid nitrogen tanks used for cell storage need to be filled with liquid nitrogen in advance