How does vapour phase cryogenic freezing work?

2023-03-03 10:14:47

Cells and sperm samples are highly proliferative and cannot be kept “active” indefinitely if they are to be used in experiments or for life research, so they are cryogenically frozen for scientific experiments and breeding.

Nor do they freeze like other objects, which need to be quick-frozen and kept active, usually with liquid nitrogen!

Cryogenic freezing of liquid nitrogen can be subdivided into two types, the gas phase and the liquid phase!

The liquid phase is simple to use. A liquid nitrogen tank is selected and filled with liquid nitrogen into which the treated sample is placed.

The gas phase, i.e. gaseous liquid nitrogen, should be used both to vaporise the liquid nitrogen and to maintain low temperatures, so how should gas phase cryogenic freezing be used?

The same liquid nitrogen tanks are used, only they are vapour phase liquid nitrogen tanks, also known as biological sample banking liquid nitrogen tanks!

This type of liquid nitrogen tank is suitable for large laboratories, or for the establishment of biological sample banks, with a large volume of liquid nitrogen and sample storage area in the inner part. An evaporator is installed at the bottom of the tank to evaporate and vaporise the liquid nitrogen into the sample storage area to maintain low temperatures.

Therefore, instead of putting cells, sperm, etc. into liquid nitrogen, the vapour phase cryogenic freezer cans can be used by simply placing them in the sample storage area. This area can be partitioned according to the type or size of sample and is easy to find at a later stage.

There is no need to worry about replenishing the liquid, as the gas phase cryogenic freezer supports automatic replenishment, simply by installing a self-pressurising liquid nitrogen tank beforehand.

How does vapour phase cryogenic freezing work