To use a liquid nitrogen container to store samples, it is inevitable to add liquid nitrogen, The new tank needs to be filled with liquid nitrogen, pre-cooled first and then filled; after storing the frozen sperm, add liquid nitrogen directly without pre-cooling!
Look at the sperm storage height!
It is consistent with the law of adding liquid nitrogen to all liquid nitrogen tanks storing biological samples!
This is to ensure that the samples in the liquid nitrogen sperm storage tank are completely submerged in liquid nitrogen. It is important to know that sperm is a highly active creature that must be highly active to be valuable, especially the sperm that are collected and stored in liquid nitrogen containers.
If there is a problem with sperm motility, there is no artificial insemination value, and it cannot be rescued. If it is caused by the untimely rehydration of the liquid nitrogen sperm storage tank, it will not be worth the loss for the user. The manufacturer of the liquid nitrogen tank recommends replenishing the sperm storage tank when the liquid nitrogen cannot cover the sample, just to avoid such problems.
In addition, it does not mean that once the liquid nitrogen cannot cover the sperm, the activity of the semen in the storage tank will be lost. This is just a more cautious approach, and it is also an effective protection for sperm storage. How long it takes depends on the volume and caliber of the liquid nitrogen sperm storage tank to determine the monitoring period, and how often to add it based on the height of the liquid nitrogen liquid level drop and the actual height of the sperm.
Now, I believe everyone knows how long it takes to add liquid nitrogen to the liquid nitrogen sperm storage tank, and why it is added in this way!