Adding liquid nitrogen is something that every user of a liquid nitrogen storage tank must experience and pay special attention to.
Whether you have a 35-liter liquid nitrogen storage tank commonly used in laboratories, a larger 65-liter or 175-liter liquid nitrogen tank for cell storage, or a 10-liter 50-liter portable liquid nitrogen container, adding liquid nitrogen during use is inevitable. of.
How many days should liquid nitrogen be added to the 35-liter liquid nitrogen storage tank commonly used in laboratories?
It needs to be determined according to your specific use method and selected caliber!
Although most of the 35-liter liquid nitrogen storage tanks used in laboratories have a diameter of 125mm, it does not mean that other diameters cannot be used. Only those with a diameter of 125mm or above can be equipped with a freezing rack. If they are used without a freezing rack, both 50mm and 80mm diameters can be used. of.
If you only store cells for embryos statically and do not open the sample after filling it with liquid nitrogen, it will not be opened until the next rehydration. Then refer to the number of days of static storage given by the manufacturer. That is, for 125mm diameter, liquid nitrogen should be added every 110 days; for 80mm diameter, liquid nitrogen should be added every 170 days; for 50mm diameter, liquid nitrogen should be added every 290 days.
There is also a 210mm caliber that is used relatively less frequently. Its static storage time is 56 days. Be sure to add liquid nitrogen around 56 days.
If your 35-liter liquid nitrogen storage tank needs to be opened regularly after loading cell embryos, once a week or twice a week. Since different users have different opening times and frequencies, in this case the manufacturer cannot give a specific reference time, and you need to do it yourself. Observe and record, from the beginning to the next rehydration, it is concluded that it is appropriate to add liquid nitrogen every few days.
These are the answers to how to add liquid nitrogen to a 35-liter liquid nitrogen storage tank every few days. For static storage, refer to the time given by the manufacturer. For dynamic storage, you need to calculate your own rehydration time.