Liquid nitrogen -196℃, It can freeze objects in contact in a short period of time. It is a good refrigerant, but it is difficult to retain when heated. Therefore, people have developed a liquid nitrogen tank, which is placed in a vacuum insulated environment to serve the sample cryopreservation. However, the liquid nitrogen tank is not static, and the samples will inevitably be put into the air. After a period of time, you will find that there is less liquid nitrogen in the tank, which is not enough. Then you need to fill it. How to fill the liquid nitrogen tank? Learn with Tianchi!
1. Funnel pouring method
Prepare a funnel, place the funnel on the target liquid nitrogen tank, pay attention to leave a certain gap between the funnel and the neck, so that the nitrogen can be discharged naturally; After that, align the mouth of the liquid nitrogen filling tank with the funnel and pour the liquid nitrogen.
2. Pressurized injection
Prepare a liquid nitrogen pump, or a self-pressurized liquid nitrogen tank. When using, open the liquid valve, connect the hose, and directly fill the liquid nitrogen tank.
What everyone needs to understand is that if your liquid nitrogen tank is used for the first time, or if it is not used for a long time, you should inject a small amount of liquid nitrogen first, observe the boiling phenomenon in the tank, and then speed up after the tank is calm. Filling speed, be careful not to let the liquid nitrogen splash, so as not to hurt yourself. At the same time, it should not be too full, at least not higher than the glass fiber reinforced plastic neck tube.