Use of cell storage tanks

2023-03-29 09:03:25

Cell storage is to store high-quality cells in a certain container after low temperature treatment, and the function and activity will not be affected within a certain period of time. This container is the cell storage tank.

The cell storage tank is designed based on the needs of cell storage. It is made of aviation aluminum alloy or 304 stainless steel, combined with a vacuum insulation structure, and uses -196°C liquid nitrogen as the medium to quickly freeze the cells without damaging their biological activity. In the absence of nitrogen, permanent preservation of cells can be achieved.

Let’s talk about the use of cell storage tanks!

Its use is mainly divided into two forms: one is to store cells in the gas phase, and the other is to store cells in the liquid phase. Different modes use different liquid nitrogen containers.

First, let’s talk about gas phase storage

Gas-phase storage is to store biological samples in a liquid nitrogen gas-phase environment, that is, in evaporated liquid nitrogen. This mode of not directly contacting liquid nitrogen can avoid tube explosion and cross-contamination.

Due to the storage of samples in liquid nitrogen partitions, the required container space and technical requirements are higher. This mode is currently only used in cell storage tanks with a volume of 350-1800 liters. It is suitable for large-scale experimental research and the establishment of cell sample banks. Stem cells, vaccines, embryos, blood and other samples are stored and used.

Second, liquid phase storage

Liquid phase storage is relative to the gas phase. The gas phase samples are stored in nitrogen, and the liquid phase cells are directly placed in liquid nitrogen after low temperature treatment, and the cryopreservation tube will be in contact with liquid nitrogen.>

This mode is used to store biological samples in liquid-phase cell storage tanks, which are common aluminum alloy liquid nitrogen containers with a volume of 1-175 liters. They are used with buckets and cryopreservation racks, which are suitable for small-batch cell storage and animal semen storage. wait.>

The above is the use of the two types of cell storage tanks. The gas-phase and liquid-phase modes have their own advantages and disadvantages. They are gas-phase storage tanks for large-scale cell storage, large space + separation, and intelligent monitoring and management system, automatic fluid replacement control, etc. A small amount of cells are stored in a liquid phase storage tank, and the cells are directly stored in liquid nitrogen, kept at a constant temperature of -196°C, and the storage is stable.

Use of cell storage tanks