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When choosing an ln2 tank, do I need to pay attention to the size of the tank mouth?

2024-05-13 09:36:58

Liquid nitrogen’s -196°C + low boiling point and quick-freezing characteristics determine that it cannot be stored in conventional containers;

This is also true. Although ordinary metal containers can withstand low temperatures, they cannot store liquid nitrogen for a long time. The only ones that can take care of both are ln2 tanks, which are made of aviation aluminum or aluminum alloy, have double internal and external gallbladders, are vacuumed, and can store liquid nitrogen for a long time. Nitrogen and can be used for samples.

Since major experimental sites, including livestock use, have various sample types and different individual storage quantities, ln2 tanks are designed in multiple models. The only difference between the models is the caliber, which is the mouth of the tank. Capacity determines the number of samples to be stored. Users who are familiar with liquid nitrogen tanks know this, but what about the mouth of the tank? Is it directly related to the sample?

When choosing an ln2 tank, do you need to pay attention to the size of the tank mouth?

Answer: Of course!

The capacity determines the amount of internal storage, and the size of the tank mouth affects the sample pickup and placement, and determines the evaporation loss of liquid nitrogen!

When the number of samples is small and the sample volume is normal, such as hundreds of semen straws and dozens of embryonic cells, in this case, the mouth of the ln2 tank has little impact on the sample pickup and placement. What needs to be considered is that the evaporation loss of large diameters is relatively high. It is generally recommended to choose a small diameter of 50mm, which is cheaper and has a longer storage time in liquid nitrogen.

However, when the number of samples is large, several thousand, special consideration needs to be given to the tank mouth. At this time, if you insist on using ln2 tanks with 50mm and 80mm tank mouths, it may be inconvenient to take the samples, and it will take a long time to take them out and put them in. , will affect the sample activity. If the cells are stored with shelves, it should be noted that only large-diameter liquid nitrogen tanks with a diameter of 125mm or above can accommodate square shelves.

There is another type where the sample volume is irregular. After processing, the sample volume is larger, such as greater than 5 cm. In this case, regardless of the number of samples, the size of the tank mouth needs to be considered to ensure that the sample can smoothly enter the inside of the ln2 tank, not just the capacity. That’s fine.

Therefore, summary: When choosing an ln2 tank, you need to pay attention to the size of the tank mouth!

When choosing an ln2 tank, do I need to pay attention to the size of the tank mouth