Hematopoietic stem cell research is a long-term topic, which may last for several months or even longer, during which samples need to be well preserved.
Good preservation means that it can neither damage the cell viability, but also can be frozen for a long time without affecting the experiment process.
The current mainstream “cold source” for cryopreservation of hematopoietic stem cells is still liquid nitrogen, and liquid nitrogen containers must be used to use liquid nitrogen!
We all know that with the development of science and technology, there are more and more types of liquid nitrogen tanks for matching laboratories and livestock farms, with different sizes, capacities, calibers, and different storage modes. Which liquid nitrogen tank is more suitable for cryopreservation of hematopoietic stem cells?
There are two types: a small vapor-liquid phase liquid nitrogen tank; and another vapor-phase Stem Cell liquid nitrogen tank!
They are both liquid nitrogen container tanks with both vapor phase and liquid phase preservation, with two zones for sample and liquid nitrogen storage in the inner part. In the case of vapor phase storage, the liquid nitrogen at the bottom can evaporate to provide a low temperature of about -190°C for the upper hematopoietic stem cells, and the cells do not touch the liquid nitrogen, so there is no risk of bursting or cross-contamination of such lyophilization tubes. They also support liquid phase, i.e. storage immersed in liquid nitrogen.
Both types of liquid nitrogen container tanks are equipped with intelligent monitoring and management system, which can view the temperature curve, can perform liquid level alarm, have data logging, support export, and can be connected to liquid nitrogen supply tank for automatic replenishment. The difference is that one of them has a small volume and the other has a large volume, one is made of aircraft aluminum and the other is made of 304 stainless steel, and both support sample partition storage.
Small vapor-liquid phase liquid nitrogen tanks if your hematopoietic stem cell count is around 2000 to 6000;
With a cell count of nearly 10,000 or over 100,000, a stainless steel vapor phase stem cell liquid nitrogen tank is better for you!
Now you know which type of liquid nitrogen container tank to choose for hematopoietic stem cell cryopreservation!