We know that the long-term preservation of biological samples such as animal sperm in liquid nitrogen tanks depends on liquid nitrogen!
The liquid nitrogen sperm storage tank provides a vacuum insulation environment, which minimizes the evaporation loss of liquid nitrogen, fully exerts its function of quick freezing without damaging the vitality of frozen sperm, and realizes long-term preservation of sperm. As for the liquid nitrogen tank, the vacuum insulation environment is jointly built by the inner and outer tank vacuum and the neck plug, which are indispensable. Depression in the tank body or a problem with the neck plug will directly affect the insulation effect.
Look at the remaining amount of liquid nitrogen in the liquid nitrogen sperm storage tank!
The remaining amount of liquid nitrogen is close to 90%, that is, not long after filling, the sperm will not be affected in a short time, but the loss of liquid nitrogen in the tank has increased significantly. If the lid problem has not been noticed, after the liquid nitrogen evaporates, the sperm motility will be gone.
The remaining amount of liquid nitrogen is less than 90%, close to 30%. Although the sperm will not die immediately, the liquid nitrogen will evaporate quickly, and the final result is the same. The sperm activity disappears, and the speed is much faster than that of liquid nitrogen close to 90%.
Because, opening the lid is equivalent to opening the liquid nitrogen sperm storage tank, allowing the liquid nitrogen to be completely placed in a normal temperature environment, similar to opening the lid of a stainless steel thermos cup, the cooling speed of the water is only better than that of ordinary water cups, and the semen that forgets to cover the lid The storage tank, after the liquid nitrogen has evaporated, the sperm will die immediately. If found before the liquid nitrogen has evaporated to 1/3 of the total capacity, the rapid rehydration can also maintain activity!
Therefore, I would like to remind everyone that when using a liquid nitrogen sperm storage tank to store frozen semen, don’t forget to cover it! In addition, note that the cover here refers to the neck plug, the plug with grooves, if it is a lock cover, the cover or not has no effect on the evaporation of liquid nitrogen!